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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in several tumors, which results in chromosome instability, and therefore may well lead to tumorigenesis. This protein was identified to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a essential purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two extra splice variants ended up noted but in depth nucleotide sequence info is not really but available.
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Blocking peptides are peptides that bind specially to your target antibody and block antibody binding. These peptide typically incorporates the epitope recognized with the antibody.
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This guideline outlines additional possible optimization of mobile and immunoassay parameters to guarantee the very best effects are...
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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin family members, whose members are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Unique cyclins exhibit distinctive expression and degradation styles which add to the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates with the G1-S section boundary and is also degraded as cells development through S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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